Figure 3. Curcumin enhanced autophagic flux and autophagosome-lysosome binding in N2a/APP695swe cells. (A) LAMP2 immunocytochemistry was used to detect the expression of lysosome in each group. Bar = 100 μm. (B) Fluorescent microscopy analysis of cells transiently overexpressing mRFP-GFP-LC3.Bar=20 μm. (C–D) Western blot analysis of P62 and LC3II in each group; Representative blot (A) and summary graph of densitometric analysis (D); The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group; **, P<0.01, compared to the APP group or DMSO group; ##, P<0.01, compared to the Bafa group).