Research Paper Volume 11, Issue 17 pp 7036—7050

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells

Figure 4. Curcumin increased the expression of retrograde axonal transport molecular motor and scaffolding proteins, decreased the expression of forward axonal transport molecular motor and promoted autophagy flux in N2a/APP695swe cells. (AF) Western blot analysis of retrograde axonal transport molecular motor DIC (AB), DHC1, DLC-3 (CD) and forward axonal transport molecular motor KIF (E-F) in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group). (GH) Western blot analysis of P62 and LC3II in each group with(out) dynein inhibitor EHNA; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group; **, P<0.01, compared to the EHNA group; ##, P<0.01, compared to the EHNA group). (IJ) Western blot analysis of scaffolding proteins Huntingtin and RILP in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.01, compared to the WT group; * P<0.001, compared to the APP or DMSO group).