Research Paper Volume 11, Issue 17 pp 7036—7050

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells

Figure 5. Curcumin promoted the expression of binding proteins dynactin (P150 and P50) and BICD2 (Bicaudal D, two-tailed D) in N2a/APP695swe cells. (AD) Western blot analysis P150, P50 and BICD2 in each group; The data represent as mean ± SEM of a typical series of 3 experiments (* P<0.01, compared to the APP or DMSO group). (EF) Western blot analysis of P150 in each group with(out) dynein inhibitor EHNA; The data represent as mean ± SEM of a typical series of 3 experiments (* P<0.01, compared to the APP or DMSO group; **, P<0.01, compared to the EHNA group; ##, P<0.01, compared to the APP or DMSO group). (G) Confocal fluorescence microscopy showed the expression of BICD2 and LC3 in each group. Bar=100/50 μm.