Research Paper Volume 11, Issue 17 pp 7123—7149

Chromobox homolog 8 (CBX8) Interacts with Y-Box binding protein 1 (YBX1) to promote cellular proliferation in hepatocellular carcinoma cells

Figure 2. CBX8 interacts with YBX1 (A) CBX8 may interact with YBX1, as visualized by BioGRID. (B) Correlation between CBX8 mRNA and YBX1 mRNA in TCGA cohort (P = 0.0001). (C) Huh7 cells were transfected with Flag-CBX8 overexpression vector for 48 h. An immunoprecipitation (IP) assay, using an anti-Flag antibody, was used to detect the binding of CBX8 and YBX1. (D) Huh7 cells were transfected with GFP-CBX8 and Flag-YBX1 overexpression vector for 48 h. An IP assay, using an anti-Flag antibody, was used to detect the binding of CBX8 and YBX1.