Research Paper Volume 11, Issue 20 pp 8745—8759

Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

Figure 6. miR-221 inhibited proliferation and fibrosis-related proteins by targeting SIRT1. (A) Directly binding region between miR-221 and SIRT1; (B) Binding relationship between miR-221 and SIRT1 was detected by luciferase reporter assay with wild or with the mutant type of the luciferase reporter plasmids of SIRT1; (CH) qPCR and western blot assays revealed that mimic-miR-221 inhibited SIRT1 expression, and inhibitor-miR-221 enhanced SIRT1 expression; (I) The efficiency of siRNA-SIRT1 was measured in MCs by qPCR; (JK) Protein expression level of Col-4 was measured by western blot in the MCs, which were transfected with inhibitor-miR-221 or siRNA-SIRT1; (LM) protein expression level of p53 was measured by western blot in the MCs, which were transfected with inhibitor-miR-221 or siRNA-SIRT1. *P < 0.05, **P < 0.01.