Figure 2. Omentin-1 deficient enhanced artery calcification in 5/6 NTP-induced mice. Six-week-old omentin-1-/- mice and their littermates were subjected to 5/6-nephrectomy or sham operation following by high phosphate diet (0.9% Pi) for indicated time. (A) Sections from the thoracic aorta of sham operation mice and 5/6 NTP mice were determined by Alizarin Red S staining and Von Kossa staining. Representative microscopic pictures were shown (upper panel) and quantity of positive staining area in the thoracic aorta were analyzed (lower panel). Scale bar 200 μm (n=6/group). (B) Calcium content of the thoracic aorta was measured in different group mice. (C). The ALP activity of the thoracic aorta was measured by an ALP kit, normalized to the total tissue protein contents. (D) Immunohistochemistry of Runx2 in the mouse thoracic aorta. Representative microscopic pictures were shown in the upper panel and quantity of positive staining area in the thoracic aorta were shown in the lower panel. Scale bar 20 μm (Red) and 500 μm (Green). Results are represented by mean ± SD with 6 replicates for each group. Significance was analyzed by two-way ANOVA with the Tukey’s HSD post hoc analysis. (*p < 0.05).