Research Paper Volume 11, Issue 17 pp 7242—7256

Reactive oxygen species-mediated senescence is accelerated by inhibiting Cdk2 in Idh2-deficient conditions

Figure 3. The senescence phenotype is promoted in Idh2 knockout mouse embryonic fibroblasts (MEFs). (A) SA-β-gal staining of wild type and Idh2 knockout MEFs. Passage 8 (P8) MEFs were used. Scale bar, 200 μm. (B) Statistical analysis of SA-β-gal-stained positive cells between wild type and Idh2 knockout MEFs. (C) BrdU level between wild type and Idh2 knockout MEFs as determined by immunocytochemistry. Nuclei were stained with DAPI, and the merged images show BrdU and DAPI signals. Scale bar, 10 μm. (D) Statistical analysis of BrdU-positive cells between wild type and Idh2 knockout MEFs. (E) Western blot analysis between wild type and Idh2 knockout MEFs. The following antibodies were used for detection: anti-Idh2, anti-p16, anti-p21, anti-p53, and anti-β-actin. Data are expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.