Research Paper Volume 11, Issue 18 pp 7510—7524

MiR-27a promotes insulin resistance and mediates glucose metabolism by targeting PPAR-γ-mediated PI3K/AKT signaling

Figure 2. MiR-27a-induced hyperglycemia amelioration and IR prevention in obese mouse model and IR cells. C57BL/6 mice were induced with HFD (Research Diet, USA; 45% kcal from fat), or with standard chow diet, both for 10 weeks. The mice were injected intravenously with AD-NC and Ad-miR-27a. (A) miR-27a expression levels in pancreas, liver, and white adipose tissue was determined using real-time PCR (B) GTT was performed in HFD-fed mice at day 7 after AD-NC or AD-miR-27a injection. (C) ITT was performed in HFD-fed mice at 7 days after AD-NC or AD-miR-27a injection. (D) In the IR 3T3-L1 cells transfected with antagomiR-27a (different doses), the miR-27a expression level was studied by qPCR. (E) Glucose levels were tested in IR 3T3-L1 cells transfected with antagomiR-27a (different doses). (F) Glucose levels were tested in IR 3T3-L1 cells transfected with antagomiR-27a (different doses) and/or supplemented with insulin. (G) 2-deoxyglucose uptake assay was also performed to detect the glucose level in both cell and cell culture medium. Number of animal per group = 8. ***P < 0.001, **P < 0.01, *P < 0.05, compared to indicated groups.