Research Paper Volume 11, Issue 20 pp 8777—8791

LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis

Figure 2. BMSC-Exos promote the protein expression of SATB2 in osteoblasts (hFOB1.19). (A) The protein expression of SATB2, Runx2, ATF4 and Hoxa2 in osteoblasts (hFOB1.19) treated with BMSC-Exos detected by Western blot analysis. (B) The protein expression of SATB2, Runx2, ATF4 and Hoxa2 in osteoblasts (hFOB1.19) treated with sh-SATB2 detected by Western blot analysis. (C) Alizarin red staining of osteoblasts (hFOB1.19) treated with sh-SATB2 (200 ×). (D) ALP staining of osteoblasts (hFOB1.19) treated with sh-SATB2 (200 ×). * p < 0.05 vs. sh-NC. Data were expressed with mean ± standard error. In Panel A and B, the unpaired t test was used for data analysis. The experiment was repeated three times.