Research Paper Volume 11, Issue 20 pp 8792—8809

Long non-coding RNA MALAT1 promotes cardiac remodeling in hypertensive rats by inhibiting the transcription of MyoD

Figure 2. LncRNA MALAT1 over-expression promotes cardiac remodeling in hypertensive rats. (A) the expression of lncRNA MALAT1 in myocardial tissues and thoracic aortic vascular tissues of SHRs after over-expression or silencing of lncRNA MALAT1 detected by RT-qPCR; B, LVW and LVW/BW ratio in SHRs after over-expression or silencing of lncRNA MALAT1; (C) Masson staining images of myocardial tissues after over-expression or silencing of lncRNA MALAT1 (scale bar = 50 μm); (D) HE staining images of myocardial tissues after over-expression or silencing of lncRNA MALAT1 (scale bar = 25 μm); (E) pathological changes of thoracic aortic vascular tissues of SHRs observed using HE staining after over-expression or silencing of lncRNA MALAT1 (× 40); (F) collagen deposition in thoracic aortic vascular tissues of SHRs after over-expression or silencing of lncRNA MALAT1, observed by Masson staining (× 400); (G) the expression of fibronectin and collagen protein in thoracic aortic vascular tissues of SHRs after over-expression or silencing of lncRNA MALAT1, determined by Western blot analysis; *, p < 0.05 vs. the LV-CON-vector group; #, p < 0.05 vs. the LV-CON-shRNA group; measurement data were expressed by means ± standard deviation and analyzed by one-way analysis of variance; n = 6.