Research Paper Volume 11, Issue 20 pp 8792—8809

Long non-coding RNA MALAT1 promotes cardiac remodeling in hypertensive rats by inhibiting the transcription of MyoD

Figure 3. Over-expression of lncRNA MALAT1 enhances ASMC proliferation and fibrosis. (A) expression of lncRNA MALAT1 in ASMCs of SHRs after over-expression or silencing of lncRNA MALAT1 detected using RT-qPCR; (B) cell activity after over-expression or silencing of lncRNA MALAT1, determined by MTT assay; (C) proliferation of ASMCs after over-expression or silencing of lncRNA MALAT1, measured by BrdU assay (× 200); (D) cell cycle analysis after over-expression or silencing of lncRNA MALAT1, determined by flow cytometry; (E) the protein expression of Bcl-2 and Bax in ASMCs after over-expression or silencing of lncRNA MALAT1 as determined by Western blot analysis; (F) the expression of fibronectin and collagen I in ASMCs after over-expression or silencing of lncRNA MALAT1, detected by Western blot analysis; *, p < 0.05 vs. the LV-CON-vector group; #, p < 0.05 vs. the LV-MALAT1-vector group; measurement data were expressed by means ± standard deviation; data in panel (AC, E and F) were analyzed by one-way analysis of variance; data in panel (D) were analyzed by repeated-measures analysis of variance; n = 3.