Research Paper Volume 11, Issue 17 pp 7294—7306

Impact of NF-κB pathway on the apoptosis-inflammation-autophagy crosstalk in human degenerative nucleus pulposus cells

Figure 3. Inhibiting NF-κB decreases apoptosis and inflammatory in LPS-induced NPCs. (A) Morphologic changes in apoptotic NP cells were stained by Hoechst 33258 (Amplification ×200). (B) Apoptotic cells were stained with Annexin V-PE and PI, and analyzed by flow cytometry. (C) Western blot analysis for the protein expression of cleaved caspase 3, Bax and Bcl-2. (D) The expression of TNF-α and IL-1β in NPCs supernatant was measured by ELISA. (E) Real-time PCR analysis for the protein expression of TNF-α and IL-1β. (F) Western blot analysis for the protein expression of TNF-α and IL-1β. Values are means ±SEM.*p<0.05 vs. control group, #p<0.05 vs. LPS group.