Figure 2. Exogenous hydrogen sulfide alleviated osteoporosis induced by Dex. (A) Serum levels of H2S, 8 weeks after treatment with GYY4137 per group. (B) 3D-image reconstruction of one representative femur per group. (C) BMD in the distal end of the intact femurs of each experimental group. (C) BMD, (D) BV/TV, (E) Tb.N, (F) Tb.Sp, and (G) Tb.Th in the distal end of the intact femurs of each experimental group. (H) Tetracycline labels observed by fluorescence light microscopy in the slices of the tibia of each experimental group. Scale bars are 50 μm. (I) Quantitative analysis of mineral apposition rate. Bone sections were fixed, decalcified, dehydrated, and sectioned. Masson trichromic staining (J) and TRAP staining (L) was shown. Scale bars are 50 μm for Masson and 200 μm for TRAP staining. Quantitative analysis of osteoblast number/bone surface (N.Ob/BS) (K) and osteoclast area/total marrow space (M). (N and O) Serum levels of CTX-I and P1NP after 8 weeks of treatment with GYY4137 in each group. n=5, *p<0.05, **p<0.01.