Research Paper Volume 11, Issue 18 pp 7553—7569

Long non-coding RNA TUG1 enhances chemosensitivity in non-small cell lung cancer by impairing microRNA-221-dependent PTEN inhibition

Figure 2. Overexpression of TUG1 enhanced the sensitivity of NSCLC cells to DDP. (A) the expression level of TUG1 after transfection of pcDNA-TUG1 and si-TUG1 detected by qRT-PCR; (B) the IC50 values of DDP in SPC-A1 cells and H520 cells transfected with si-TUG1 and SPC-A1/DDP and H520/DDP cells transfected with pcDNA-TUG1; (C) colony forming ability of transfected cells in each group under DDP treatment detected by colony formation assay; (D) migration ability of transfected cells in each group under DDP treatment detected by scratch test; (E) the invasive ability of transfected cells in each group detected by Transwell assay; ** p < 0.01 versus the si-NC group.