Research Paper Volume 11, Issue 18 pp 7553—7569

Long non-coding RNA TUG1 enhances chemosensitivity in non-small cell lung cancer by impairing microRNA-221-dependent PTEN inhibition

Figure 5. Relationship between TUG1 and miR-221 and their effects on chemoresistance in NSCLC. (A) the expression level of miR-221 in the sensitive group and the resistant group and the relationship with TUG1; (B) the dual luciferase reporter assay to verify the targeting relationship of TUG1 and miR-221; (C) RIP analysis with the Ago2 antibody, and then quantitative analysis of TUG1 and miR221 by qRT-PCR; (D) colony formation to detect the effect of TUG1 and miR-221 on cell colony formation ability; (E) scratch test to detect the effect of TUG1 and miR-221 on cell migration ability; (F) Transwell invasion assay to detect the effect of TUG1 and miR-221 on the cell invasive ability; (G) flow cytometry to detect the effects of TUG1 and miR-221 on cell cycle and apoptosis; (H) expression of autophagy-related proteins and cell senescence-related protein hTERT determined by Western blot analysis, normalized to β-actin. * p < 0.05 versus the control group; ** p < 0.01; # p < 0.05 versus the si-TUG1 group; & p < 0.05 versus the TUG1 group.