Research Paper Volume 11, Issue 18 pp 7553—7569

Long non-coding RNA TUG1 enhances chemosensitivity in non-small cell lung cancer by impairing microRNA-221-dependent PTEN inhibition

Figure 7. Overexpression of TUG1 promotes sensitivity of NSCLC cells by positively regulating PTEN. (A) colony formation assay to detect the effect of TUG1 and PTEN on cell colony formation ability; (B) scratch test to detect the effect of TUG1 and PTEN on cell migration ability; (C) Transwell invasion assay to detect the effect of TUG1 and PTEN on cell invasion ability; (D) flow cytometry to detect the effect of TUG1 and PTEN on cell cycle; (E) flow cytometry to detect the effect of TUG1 and PTEN on cell apoptosis; (F) expression of autophagy-related proteins and cell senescence-related protein hTERT determined by Western blot analysis, normalized to β-actin. * p < 0.05 versus the NC group; ** p < 0.01.