Figure 4. PTPN1 and MAP3K11 are up-regulated and negatively correlated with the expression of miR-125a-5p in HCC. (A and B) PTPN1 and MAP3K11 were up-regulated in TCGA in Starbase. (C and D) High expression of PTPN1 and MAP3K11 was associated with poorer overall survival in the GEPIA database. (E and F) qRT-PCR indicated that PTPN1 and MAP3K11 were up-regulated in the eight matched pairs of HCC tissues compared to the adjacent normal tissues. Student’s t-test, mean ± SD, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. (G–I) qRT-PCR and Western blots indicated that PTPN1 and MAP3K11 were up-regulated in HCC cell lines. One-way ANOVA, mean ± SD, *P<0.05, **P<0.01. (J and K) Spearman correlation analysis showed that miR-125a-5p expression was negatively correlated with PTPN1 and MAP3K11 expression.