Research Paper Volume 11, Issue 18 pp 7723—7745

Neural stem cell small extracellular vesicle-based delivery of 14-3-3t reduces apoptosis and neuroinflammation following traumatic spinal cord injury by enhancing autophagy by targeting Beclin-1

Figure 1. NSC-sEVs transport 14-3-3t into neuronal cells and rat spinal cord tissue. (A) Western blot was used to detect the expression of 14-3-3t in neuronal cells. (B) Semi-quantitative detection of 14-3-3t proteins expression level in neurons, normalized to GAPDH. (C) Western blot analysis of 14-3-3t expression in rat spinal cord tissue (D) Semi-quantitative detection of 14-3-3t proteins expression level in spinal cord tissues, normalized to GAPDH. (E) Western blotting was used to detect the expression of 14-3-3t and GAPDH in NSC-sEVs. * p < 0.05, compared to the Glu or SCI group. NSC-sEVs, neural stem cell-derived small extracellular vesicles; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Glu, glutamate; SCI, spinal cord injury.