Research Paper Volume 11, Issue 18 pp 7780—7795

Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer

Figure 1. SIP-SII impairs proliferation and migration and attenuates Akt signaling in bladder cancer cells. (A) Half-maximum inhibitory concentration (IC50) of test drugs evaluated through the MTT viability assay. (B) Cell viability (MTT) assay results for RT112 and JMSU1 cells treated respectively with 2.5 μM or 5μM SIP-SII for the indicated time-points. Cell migration assay results for RT112 cells (C) and JMSU1 cells (D) treated respectively with 2.5 μM or 5.0 μM SIP-SII for 24 h. Representative images at 200x magnification. Data are mean ± SD (error bars) of three experiments performed in triplicate. **P < 0.01 vs. DMSO (control); n = 3. (E) Western blot analysis of total Akt, phospho-Akt, CDK4, Bcl-2, and MMP2 24 h post-exposure to SIP-SII (RT112: 2.5 μM; JMSU1: 5.0 μM).