Figure 3. Overexpression of Akt DD abrogates SIP-SII inhibitory effects. (A) Cell viability assay results for RT112 and JMSU1 cells treated with the FLAG-HA empty vector and DMSO (control), 5.0 μM SIP-SII, or SIP-SII combined with Akt DD (PKB T308D S473D) for 24 h. Cell migration assay results for RT112 (B) and JMSU1 cells (C) treated with SIP-SII alone or in combination with Akt DD for 24 h. Representative images at 200x magnification. Data are mean ± SD (error bars) of three experiments performed in triplicate. **P < 0.01 vs. DMSO; n = 3. Western blot analyses in RT112 (D) and JMSU1 (E) cells treated with SIP-SII alone or in combination with Akt DD for 24 h.