Research Paper Volume 11, Issue 18 pp 7780—7795

Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer

Figure 4. Inhibition of cell proliferation and migration by dual treatment with SIP-SII and AZD4547. (A) Results of cell viability assays in RT112 and JMSU1 cells cultured with DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or SIP-SII and AZD4547 combined. Transwell migration assay results for RT112 (B) and JMSU1 (C) cells treated with DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or the combination of SIP-SII and AZD4547 for 24 h. Representative images at 200x magnification. Cell cycle analyses of RT112 (D) and JMSU1 (E) cells treated with DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or dual treatment with SIP-SII and AZD4547. Flow cytometry was performed 24 h post-treatment in PI-stained cells. JC-1 apoptosis assay results in RT112 (F) and JMSU1 (G) cells treated (24 h) with DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or the combination of SIP-SII and AZD4547. Data are mean ± SD (error bars) of three individual experiments. **P < 0.01 vs. DMSO; ##P < 0.01 vs. SIP-SII; ^^P < 0.01 vs. AZD4547; n = 3.