Figure 5. Dual treatment with SIP-SII and AZD4547 potentiates the inhibition of Akt and downstream effectors. (A) Western blot analysis of RT112 and JMSU1 cells exposed (24 h) to DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or SIP-SII combined with AZD4547. (B) Western blot analysis of RT112 and JMSU1 cells treated for 24 h with DMSO, 5.0 μM SIP-SII, 100 nM AZD4547, or SIP-SII combined with AZD4547 in the presence of 0.5 μM BKM120. Akt in, Akt inhibitor BKM120. ** vs. Con, ## vs. AKT IN, ^^ vs. AKT IN+SIP, $$ vs. AKT IN+AZD. **, ##, ^^ and $$ indicated P < 0.01, n=3. (C) Cell viability assay results. (D) Transwell migration assay results. Representative images at 200x magnification. Data are mean ± SD (error bars) of three individual experiments. **P < 0.01 vs. control; n = 3.