Research Paper Volume 11, Issue 18 pp 7830—7846

Downregulation of LncRNA-XIST inhibited development of non-small cell lung cancer by activating miR-335/SOD2/ROS signal pathway mediated pyroptotic cell death

Figure 2. The effects of LncRNA-XIST on NSCLC cell proliferation. (A) Real-Time qPCR was used to detect LncRNA-XIST levels in A549 cells. (B) Cell counting assay was used to count A549 cell numbers. (C) CCK-8 kit was used to evaluate A549 cell proliferation. (D) Real-Time qPCR was used to detect LncRNA-XIST levels in H1299 cells. (E) Cell counting assay was used to count H1299 cell numbers. (F) CCK-8 kit was used to evaluate H1299 cell proliferation. (G) Real-Time qPCR was used to detect LncRNA-XIST levels in HBE cells. (H) Cell counting assay was used to count HBE cell numbers. (I) CCK-8 kit was used to evaluate HBE cell proliferation. (J) Western Blot was used to detect cell cycle associated proteins (Cyclin D1, Cyclin E2, CDK2, CDK4 and CDK6), which was normalized to β-actin and (K) quantified by Image J software. (“NS” represented no statistical significance, “*” represented p < 0.05, “**” represented p < 0.01).