Research Paper Volume 11, Issue 18 pp 7914—7937

TIP-B1 promotes kidney clear cell carcinoma growth and metastasis via EGFR/AKT signaling

Figure 5. TIP-B1 trigger epithelial-mesenchymal transition by activating the AKT pathway. (A) GSEA analyses detected the tight junction markers were enriched in the TIP-B1 low group. (BC) Expressions of EMT-related markers in KIRC cells with TIP-B1 downregulation by RT-PCR (B) and WB (C) assays. (D) Western blot assays detect candidate signal pathway. (E) GSEA analyses detected the AKT signal were enriched in TIP-B1 high group. (FH) Correlation analysis between TIP-B1 and AKT1 (F), AKT2 (G), PTEN (H) in TCGA database. (IJ) Proliferation in the in OSRC-2 (I) and 786-O (J) cell lines was evaluated by CCK-8 assay. (K) Expressions of EMT-related markers in the indicated KIRC cells were detected by western blot assay. (L-O) Transwell migration (L, N) and invasion (M, O) assay was evaluated in indicated groups.