Research Paper Volume 11, Issue 18 pp 7914—7937

TIP-B1 promotes kidney clear cell carcinoma growth and metastasis via EGFR/AKT signaling

Figure 6. TIP-B1/EGFR axis modulates AKT signaling in KIRC. (A) GSEA analyses detected the receptor agonist activity were enriched in TIP-B1 high group. (B) Western blot assays detect the phosphorylation levels of AKT in OS-RC-2 (left) or 786-O cells (right) treated with EGF. (C) Western blot assays detect the phosphorylation levels of AKT and EGFR treated with EGF. (D) Western blot assays detect the phosphorylation levels of AKT and EGFR treated with EGF and EGFR specific inhibitor (AG1478). (E) Western blot assays detect EGFR with cycloheximide (F) Coimmunoprecipitation by TIP-B1(left) and EGFR (right) in786-O cells.