Research Paper Volume 11, Issue 20 pp 8845—8859

Figure 3. The effect of COL6A6 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells. (A) The expression of COL6A6 protein was determined by western blotting; (B) The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; (C and D) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40×); (E–G) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4×); (H–K) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. ^*P<0.05, ^**P<0.01, ^***P<0.001, compared with the NC group.