Research Paper Volume 11, Issue 20 pp 8845—8859

COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway

Figure 3. The effect of COL6A6 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells. (A) The expression of COL6A6 protein was determined by western blotting; (B) The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; (C and D) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40×); (EG) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4×); (HK) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. *P<0.05, **P<0.01, ***P<0.001, compared with the NC group.