Research Paper Volume 11, Issue 20 pp 8845—8859

COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway

Figure 6. The effect of COL6A6 interacted with P4HA3 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells through regulating the PI3K-Akt pathway. (A and B: The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; (C and E) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40×); (D and F) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4×); (G) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. *P<0.05, **P<0.01, ***P<0.001, compared with NC group; #P<0.05, ##P<0.01, ###P<0.001, compared with COL6A6 group.