Research Paper Volume 11, Issue 20 pp 8860—8878

Long non-coding SBF2-AS1 acting as a competing endogenous RNA to sponge microRNA-142-3p to participate in gemcitabine resistance in pancreatic cancer via upregulating TWF1

Figure 1. Expression of SBF2-AS1 in pancreatic cancer tissues and cells. (A) Detection of SBF2-AS1 expression in pancreatic cancer and adjacent normal tissues by RT-qPCR, N = 82. (B) Detection of SBF2-AS1 expression in pancreatic cancer cells and normal cells by RT-qPCR. (C) Bioinformatics analysis to predict the expression localization of SBF2-AS1. (D) Detection of expression localization of SBF2-AS1 by nuclear and cytoplasmic separation assay. (E) FISH experiment to verify the expression localization of SBF2-AS1. Repetitions = 3; Data was analyzed using the t test or one-way ANOVA. * P < 0.05 vs HPDE6-C7 cells.