Research Paper Volume 11, Issue 20 pp 8860—8878

Long non-coding SBF2-AS1 acting as a competing endogenous RNA to sponge microRNA-142-3p to participate in gemcitabine resistance in pancreatic cancer via upregulating TWF1

Figure 2. Identification of drug resistance and expression of SBF2-AS1 in AsPC-1/GEM and PANC-1/GEM cells. (A) Identification of drug resistance in AsPC-1 and PANC-1, AsPC-1/GEM and PANC-1/GEM cells by MTT assay. (B) Detection of the expression of SBF2-AS1 in AsPC-1 and PANC-1, AsPC-1/GEM and PANC-1/GEM cells by RT-qPCR. (C) RT-qPCR to detect transfection efficiency of SBF2-AS1 siRNA. Repetitions = 3; Data was analyzed using the t test or one-way ANOVA. * P < 0.05 vs parent cells with same concentration.