Research Paper Volume 11, Issue 20 pp 8860—8878

Long non-coding SBF2-AS1 acting as a competing endogenous RNA to sponge microRNA-142-3p to participate in gemcitabine resistance in pancreatic cancer via upregulating TWF1

Figure 3. Effect of knock-down of SBF2-AS1 expression on proliferation, cell cycle distribution and apoptosis of drug-resistant pancreatic cancer cells. (A) Determination of cell proliferation of AsPC-1/GEM and PANC-1/GEM cells by MTT assay. (B) EdU assay used to detect DNA replication activity in AsPC-1/GEM and PANC-1/GEM cells (× 200). (C) Detection of colony formation ability of AsPC-1/GEM and PANC-1/GEM cells in each group by colony formation experiment. (D) Flow cytometry utilized to detect cell cycle distribution of AsPC-1/GEM and PANC-1/GEM cells. (E) Flow cytometry was utilized to detect cell apoptosis of AsPC-1/GEM and PANC-1/GEM cells. Repetitions = 3; Data was analyzed using the t test or one-way ANOVA.