Figure 9. Inhibition of miR-127-3p and miR-125b-5p decreased M1 macrophage polarization. BMDMs were transfected with miR-127-3p inhibitor/control RNA or miR-125b-5p inhibitor/control RNA and stimulated with or without LPS (100 ng/ml) 6 h later. (A) Confirmation of successful transfection of miR-127-3p inhibitor and miR-125b-5p inhibitor was determined via qRT-PCR. Data are presented as mean ± SEM, n = 4-5. *p < 0.05, **p < 0.01. (B) Levels of the M1 markers IL-12 and CCR-7 in BMDMs at 24 h after LPS treatment were analyzed via qRT-PCR. Data are presented as mean ± SEM, n = 6–10. *p < 0.05, **p < 0.01.