Figure 5. Effect of LY with or without irradiation on the gene expression of GC cell lines. (A) Western blot analysis: representative results of the TGF-β1, TGF-β2, SMAD4,E-CA, N-CA, IL-1β, IL-6, PD-L1 and γ-H2AX expressions in GC cells after LY with or without irradiation treatment. (B) ELISA: the expression of TGF-β1, TGF-β2 and IL-6 in culture medium of GC cells after LY with or without irradiation treatment. (C) Western blot analysis: the protein stability of TGF-β1 and TGF-β2 regulated in response to irradiation. All data represent three independent experiments, mean ± SEM, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.