Research Paper Volume 11, Issue 20 pp 8911—8924

Immature dendritic cells derived exosomes promotes immune tolerance by regulating T cell differentiation in renal transplantation

Figure 4. miR-682 was highly expressed in imDEC which suppressed the IL17+CD4+T cells and promoted the Foxp3+CD4+T cells. (A) According to the information of GSE33179, the expressions of miR-682, miR-649, miR-805 and miR-467f in imDEC and mDEC. (B) qRT-PCR detected the expressions of miR-682, miR-649, miR-805 and miR-467f in imDEC and mDEC. *P<0.05 vs mature exosome; **P<0.01 vs mature exosome. (C) Under anti-CD3/CD28 activated condition, mRNA level of IL-17A and mRNA level of Foxp3 were detected in control, miR-682 mimic and miR-805 mimic groups. ROCK2, p-STAT3 and p-STAT5 expressions in control, miR-682 mimic and miR-805 mimic groups. *P<0.05 vs control; **P<0.01 vs control. (D-E) The expression of STAT5, STAT3 and ROCK2 were measured in control, miR-682 mimic and miR-805 mimic groups. (F) Under anti-CD3/CD28 activated condition, mRNA level of IL-17A and mRNA level of Foxp3 were detected in control-sponge-imDEC, miR-682-sponge-imDEC and miR-805-sponge-imDEC groups. (G) The expression of STAT5, STAT3 and ROCK2 were measured in control-sponge-imDEC, miR-682-sponge-imDEC and miR-805-sponge-imDEC groups. The expression of p-STAT5, p-STAT3 and ROCK2 were measured in control-sponge-imDEC, miR-682-sponge-imDEC and miR-805-sponge-imDEC groups. **P<0.01 vs control-sponge-imDEC.