Figure 3. Zinc pre-treatment increased the expression levels of Nrf2 and its downstream target genes in testicular tissues. (A) Immunohistochemical Staining showed Nrf2 and NF-κB expression levels in Control, I/R injury and I/R injury treated with Zinc groups. (B) Statistical analyses of IHC results in Nrf2 and NF-κB expression levels in different groups. (C) Protein levels of Nrf2, NF-κB, HO-1, NQO-1 and GST in different groups. Histone H3 and β-actin were used as a protein control to normalize volume of protein expression. (D) Statistical analyses of Western Blotting results in Nrf2, NF-κB, HO-1, NQO-1 and GST protein levels in different groups. Data are expressed as mean± SD. *significant difference vs. Control group (P< 0.05); #significant difference vs. I/R group (P< 0.05).