Figure 4. Differentially expressed miRNAs in testicular tissues of rats induced by I/R injury based on microarrays. (A) Hierarchical clustering analysis of the differentially expressed miRNAs between Control and I/R injury testis samples in rats. (B) A schematic diagram used to search the target miRNAs of Nrf2 in three databases. (C) Validation of the five differently expressed miRNAs in rats caused by I/R injury based on real-time PCR assay. (D) MiRNA-101-3p expression level of TM3 after transfecting miR-101-3p mimics and inhibitor. (E) Nrf2 expression level of TM3 after transfecting miR-101-3p mimics and inhibitor. All data are expressed as the mean ± SD. *significant difference vs. Control group (P< 0.05).