Research Paper Volume 11, Issue 21 pp 9295—9309

Zinc-induced protective effect for testicular ischemia-reperfusion injury by promoting antioxidation via microRNA-101-3p/Nrf2 pathway

Figure 6. Transfecting miR-101-3p mimics and inhibitor regulated H/R-induced oxidative damage in vitro. (A) MiRNA-101-3p expression level of TM3 in H/R group after transfecting miR-101-3p mimics and inhibitor. (B) The intracellular ROS and Nrf2 of TM3 in H/R group after transfecting miR-101-3p mimics and inhibitor. (C) Protein levels of Nrf2, NF-κB, HO-1, NQO-1 and GST of TM3 after transfecting miR-101-3p mimics and inhibitor. Histone H3 and β-actin were used as a protein control to normalize volume of protein expression. Data are expressed as mean± SD. *significant difference vs. Control group (P< 0.05).