Research Paper Volume 11, Issue 21 pp 9295—9309

Zinc-induced protective effect for testicular ischemia-reperfusion injury by promoting antioxidation via microRNA-101-3p/Nrf2 pathway

Figure 7. The interaction between miR-101-3p and Nrf2 in TM3. (A) Protein levels and statistical analyses of Western blotting results of Nrf2 in TM3 which administrated with si-Nrf2. (B) Cell viability of TM3 in different treated groups. (C) Protein levels of Nrf2, NF-κB, HO-1, NQO-1 and GST of TM3 in different groups. Histone H3 and β-actin were used as a protein control to normalize volume of protein expression. (D) Statistical analyses of Western blotting results in Nrf2, NF-κB, HO-1, NQO-1 and GST expression levels in different groups. Data are expressed as mean±SD. *significant difference vs. Control group (P< 0.05); #significant difference vs. H/R+NC inhibitor+si-Control group (P< 0.05); &significant difference vs. H/R+miR-101-3p inhibitor+si-Control group (P< 0.05).