Research Paper Volume 11, Issue 20 pp 8998—9012

TIMP1 mRNA in tumor-educated platelets is diagnostic biomarker for colorectal cancer

Figure 6. TEPs promote CRC cell proliferation and inhibit cancer cell apoptosis. (AB) The protein level of TIMP1 in platelets from HV and CRC by ELISA (A) and western blotting (B). (CD) The mRNA (C) and protein (D) level in HT29 cells incubated with platelets from CRC patients and HVs. (E) The proliferation of HT29 cells exposed to platelets from HVs and colorectal cancer patients were determined by CCK-8 assays. (F) Apoptosis of HT29 cells exposed to platelets from healthy volunteers and colon cancer patients for 72 h were determined by flow cytometry. (GH) The mRNA (G) and protein (H) level in Caco-2 cells incubated with platelets from CRC patients and HVs. (I) The proliferation of Caco-2 cells exposed to platelets from HVs and colon cancer patients were determined by CCK-8 assays. (G) Apoptosis of Caco-2 cells exposed to platelets from HVs and colon cancer patients for 72 h were determined by flow cytometry. **P < 0.01, ***P < 0.001.