Research Paper Volume 11, Issue 20 pp 9075—9089

Sirtuin 3 attenuates neuroinflammation-induced apoptosis in BV-2 microglia

Figure 3. Sirt3 inhibits LPS-induced mitochondrial apoptosis. (A, B) ROS production was measured via immunofluorescence. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. (C, E) Levels of cellular antioxidants were determined via ELISA. (F) mPTP opening rate was measured in response to LPS treatment and Sirt3 overexpression. (G, H) Immunofluorescence assay for Smac. Levels of nuclear Smac expression were determined in BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus. (I) Caspase-9 activity was detected via ELISA. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group. N=3 independent experiments.