Research Paper Volume 11, Issue 20 pp 9075—9089

Sirtuin 3 attenuates neuroinflammation-induced apoptosis in BV-2 microglia

Figure 4. SRV2-associated mitochondrial fission is activated by LPS and inhibited by Sirt3. (A, B) Mitochondrial fission was measured via immunofluorescence. Numbers of fragmented mitochondria were recorded in BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus. (CH) Transcription of mitochondrial fission-related factors. RNA was isolated from BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus. qPCR was used to measure changes in levels of mitochondrial fission-related proteins. (I) SRV2 expression was measured via qPCR. (J) Mitochondrial fission was measured in BV-2 cells transfected with SRV2 adenovirus via immunofluorescence. Numbers of fragmented mitochondria were recorded in BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group. N=3 independent experiments.