Research Paper Volume 11, Issue 20 pp 9075—9089

Sirtuin 3 attenuates neuroinflammation-induced apoptosis in BV-2 microglia

Figure 6. Sirt3 modulates SRV2-associated mitochondrial fission via the Mst1-JNK pathway. (AC) Immunofluorescence assay for Mst1 and p-JNK. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. (D) RNA was isolated from BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus. qPCR was then used to measure changes in SRV2 levels. Ani, an agonist of the Mst1-JNK pathway, was used to re-activate its activity. (E, F) Mitochondrial fission was measured via immunofluorescence. Numbers of fragmented mitochondria in BV-2 cells treated with LPS and/or transfected with Sirt3 adenovirus were recorded. Ani was used to activate the Mst1-JNK pathway. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group; @P<0.05 vs. LPS+adenovirus-Sirt3 group. N=3 independent experiments.