Research Paper Volume 11, Issue 21 pp 9530—9543

Proteome and phosphoproteome reveal mechanisms of action of atorvastatin against esophageal squamous cell carcinoma

Figure 1. Atorvastatin suppresses ESCC cells proliferation and anchor-independent cell growth. (A) KYSE150 cells (left panel) and KYSE450 cells (right panel) were treated with atorvastatin (0, 1, 2.5, 5 and 10 μM). Cell number was counted at 0, 24, 48, 72, 96 h by analysis at IN Cell Analyzer 6000. Data are shown as means ± SD (* P < 0.05 *** P < 0.001 vs. untreated control, n ≥ 3). (B) Atorvastatin effectively inhibits anchorage-independent cell growth. KYSE150 and KYSE450 cells (8 ×103/well) were exposed to different concentrations of atorvastatin with 1.25% Basal Medium Eagle agar containing 10% FBS and cultured for 8 days. Colonies were counted for analysis by IN Cell Analyzer 6000 soft agar program. Data are shown as means ± SD, (* P < 0.05, ** P < 0.01 *** P < 0.001 vs. untreated control, n ≥ 3).