Research Paper Volume 11, Issue 21 pp 9581—9596

Long non-coding RNA PVT1 encapsulated in bone marrow mesenchymal stem cell-derived exosomes promotes osteosarcoma growth and metastasis by stabilizing ERG and sponging miR-183-5p

Figure 4. PVT1 in BMSC-EXO promotes osteosarcoma cell proliferation and migration via increasing ERG. (A) The BMSC-EXOsi-PVT1 was co-cultured with MNNG/HOS cells for 48 h. After the co-culturing, cell proliferation and migration were detected using CCK-8, colony formation, scratch and Transwell migration assays. (B) The MNNG/HOSsi-ERG cells and MNNG/HOSsi-Ets-1 cells were co-cultured with or without BMSC-EXO for 48 h. The cell proliferation and migration were detected. (C) The BMSC-EXOsi-PVT1 was co-cultured with MNNG/HOS cells or MNNG/HOSAd-ERG cells for 72 h. The cell proliferation, migration, and invasion were detected. Three independent experiments. *p<0.05, **p<0.01 vs negative control or exosomes+si-control or si-control. #p<0.05, ##p<0.01 vs exosomes+si-control or si-PVT1+Ad-GFP. BMSC-EXOsi-PVT1, exosomes isolated from PVT1-interfering BMSC. MNNG/HOSsi-ERG cells, the MNNG/HOS cells which were transfected with siRNA of ERG. MNNG/HOSsi-Ets-1 cells, the MNNG/HOS cells which were transfected with siRNA of Ets-1. MNNG/HOSAd-ERG, the MNNG/HOS cells which were transfected with ERG-overexpressing vector. CCK-8, cell counting kit-8 assay.