Research Paper Volume 11, Issue 21 pp 9626—9642

Cellular senescence induced by S100A9 in mesenchymal stromal cells through NLRP3 inflammasome activation

Figure 6. IL-1β is sufficient to induce cellular senescence. (A) Cells were treated with rhIL-1β (10 ng/ml) for 72h. Next, senescent cells were counted and presented in graphs (40 × magnification). The levels of p53, p21, and p16 were measured using qPCR and western blot. (B) RNAi efficiency of IL-1β was assayed utilizing qPCR and western blot. (C) IL-1β was knocked down, and cells were treated with S100A9 for 72h, senescent cells were counted and presented in graphs (40 × magnification). The expression of p53, p21 and p16 was determined using qPCR and western blot. Data are expressed as mean ± SD of three experiments. *P<0.05, **P<0.01, and ***P<0.001.