Research Paper Volume 11, Issue 21 pp 9689—9708

Circular RNA circGRAMD1B inhibits gastric cancer progression by sponging miR-130a-3p and regulating PTEN and p21 expression

Figure 2. Overexpression of circGRAMD1B inhibits the proliferation, migration, and invasion of GC cells. (A) qRT-PCR analysis of the transfection efficiency of circGRAMD1B in MGC803 and SGC7901 cells. (BC) Cell proliferation abilities were detected by CCK-8 and colony formation assays after the transfection of circGRAMD1B or vector in MGC803 and SGC7901 cells. (D) Observation of DNA synthesis in MGC803 and SGC7901 cells transfected with circGRAMD1B overexpressing plasmid or control vector by the EdU assay. (E) Cell migration and invasion abilities were measured with transwell assays after the transfection of circGRAMD1B or vector in MGC803 and SGC7901 cells. (F) Cell migration ability was determined by a wound-healing assay after the transfection of circGRAMD1B or vector in MGC803 and SGC7901 cells. (Values are shown as the mean ± standard error of the mean based on three independent experiments. *P < 0.05, **P < 0.01).