Research Paper Volume 11, Issue 21 pp 9689—9708

Circular RNA circGRAMD1B inhibits gastric cancer progression by sponging miR-130a-3p and regulating PTEN and p21 expression

Figure 3. Silencing of circRAMD1B promotes the proliferation, migration, and invasion of GC cells. (A) Schematic representation of siRNA1/siRNA2 designed to target circGRAMD1B at the back splice junctions. (B) qRT-PCR analysis of the silencing efficiency of circGRAMD1B after the transfection for 48 h in AGS and BGC823 cells. (C) CCK-8 analysis of the cell viability after the transfection of si-circGRAMD1B or si-NC in AGS and BGC823 cells. (D) Colony formation analysis of the cell colony number after the transfection of si-circGRAMD1B or si-NC in AGS and BGC823 cells. (E) EdU analysis of the DNA synthesis after the transfection of si-circGRAMD1B or si-NC in AGS and BGC823 cells. (F) Transwell analysis of the cell migration and invasive potential after the transfection of si-circGRAMD1B or si-NC in AGS and BGC823 cells. (Values are shown as the mean ± standard error of the mean based on three independent experiments. *P < 0.05, **P < 0.01).