Research Paper Volume 11, Issue 22 pp 10061—10073

PGC1α activation by pterostilbene ameliorates acute doxorubicin cardiotoxicity by reducing oxidative stress via enhancing AMPK and SIRT1 cascades

Figure 3. Effect of pterostilbene treatment combined with SIRT1 siRNA on cell viability, ATP content, ROS generation and ΔΨm, and SIRT1 and PGC1α signaling in DOX-treated H9c2 cells (24 h). (A) and (B) Representative western blot results of SIRT1, PGC1α, Ac-PGC1α, UCP2 and NRF1 are shown. Membranes were re-probed for β-actin expression to show that similar amounts of protein were loaded in each lane. IB, immunoblot; IP, immunoprecipitation. (C) Cell viability, (D) cellular ATP content, (E) ROS level and (F) ΔΨm are shown, and (CE) three indexes in the Control group of siCON are defined as 100%. The results are expressed as the mean ± SEM. *P < 0.05 vs. the Control group, #P < 0.05 vs. the 1 μM DOX-treated group, $P < 0.05 vs. the siCON group with the representative same drug treatments.