Research Paper Volume 11, Issue 22 pp 10061—10073

PGC1α activation by pterostilbene ameliorates acute doxorubicin cardiotoxicity by reducing oxidative stress via enhancing AMPK and SIRT1 cascades

Figure 5. Effect of pterostilbene treatment combined with Compound C or EX527 on AMPK, SIRT1 and PGC1α signaling protein levels in DOX-stimulated mice hearts. Representative western blot results of p-AMPK, AMPK, SIRT1, PGC1α, Ac-PGC1α, UCP2 and NRF1 are shown. Membranes were re-probed for Tubulin expression to show that similar amounts of protein were loaded in each lane. IB, immunoblot; IP, immunoprecipitation. The results are expressed as the mean ± SEM. *P < 0.05 vs. the Sham group, #P < 0.05 vs. the DOX-treated group, $P < 0.05 vs. the DOX+PTS cotreated group.