Research Paper Volume 11, Issue 21 pp 9811—9828

The TORC1 inhibitor Nprl2 protects age-related digestive function in Drosophila

Figure 2. Nprl2 plays important roles in Drosophila digestive function. (A and B) Newly hatched yw and nprl21 flies were cultured on standard food for the indicated number of days and then dissected. (A) Crop sizes were grouped into four classes based on area (mm2). The ratio of different crop sizes was quantified. Error bars represent the SD from four independent experiments. (B) The ratio of flies with food content in their crops was quantified. Error bars represent the SD from four independent experiments. (C) 15 day-old flies were cultured on blue dyed food for 3 days and then transferred to standard food for the indicated time. The ratio of flies with blue dye in the digestive tube at each time point was calculated. Error bars represent the SD from three independent experiments. **P < 0.01, ***P < 0.001. N is the total number of flies used.