Research Paper Volume 11, Issue 21 pp 9875—9892

Promising therapeutic effect of thapsigargin nanoparticles on chronic kidney disease through the activation of Nrf2 and FoxO1

Figure 3. The cell viability and UPR-related protein expression after TG NP treatment in HK-2 cells. (A) Cell viability was analyzed by SRB assays. Cells were treated with different concentrations of the TG NPs for 24 h. *p < 0.05 versus the control. (B) UPR-related protein expression was measured by western blot analysis. Cells were treated with different concentrations of the TG NPs for 24 h. (C) Cell viability after H2O2 treatment was analyzed by SRB assays. Cells were treated with different concentrations of H2O2 for 18 h. *p < 0.05 versus the control. (D) TG NPs showed cytoprotection against oxidative stress. HK-2 cells were pretreated with or without the TG NPs for 6 h and then incubated with H2O2 for 18 h. *p < 0.05 versus the H2O2 group.